FS5 Spectrofluorometer

FS5

SPECTROFLUOROMETER

Compact high-sensitivity spectrofluorometer; FS5 v2 is adaptable to a wide range of research and analytical applications.

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Sample Options

MicroPL Upgrade

Dedicated Team

Multiple Lifetime Measurements

Highly Sensitive

NIR Upgrade

PRODUCT DESCRIPTION

The FS5 Spectrofluorometer is a compact, high-performance benchtop system designed for the comprehensive characterisation of fluorescent and phosphorescent samples. Since its launch in 2013, the FS5 series has become a trusted solution for fluorescence research, contributing to thousands of scientific publications worldwide.

The FS5 v2 Spectrofluorometer is the latest evolution of the FS5, offering enhanced performance and expanded capabilities to meet the growing demands of modern photoluminescence spectroscopy.

Equipped with top-tier optical components, photon-counting sensitivity, and fluorescence lifetime capabilities, the FS5 excels in both rapid routine analysis and sensitive research tasks.

This compact benchtop instrument adapts seamlessly to your needs, offering flexibility with multiple source and detector configurations without expanding its footprint. As standard, the FS5 v2 includes a photon-counting PMT detector for visible-range fluorescence spectra and an absorption detector. It can be easily upgraded to perform NIR measurements up to 1650 nm, time-resolved fluorescence or phosphorescence, photoluminescence quantum yields, and anisotropy.

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PRODUCT DETAILS

SOFTWARE

Fluoracle® is the operating software for the FS5 Spectrofluorometer. It controls all FS5 steady state and time-resolved spectrometer features with a straightforward design concept: focus on all modern photoluminescence spectroscopy applications and provide a user-friendly interface with ‘ready to publish’ outputs.

Figure 1: Fluoracle software interface

Whether you select a basic spectral scanning version of FS5 Spectrofluorometer, or you go for an advanced version that includes TCSPC lifetime measurements or integrating sphere measurements, the all-inclusive software will provide instrument options automatically, from data acquisition to analysis and presentation.

Upgrade to FAST Software for advanced fluorescence lifetime analysis.

MEASUREMENT EXAMPLES

The FS5 Spectrofluorometer can measure emission spectra up to 1650 nm when equipped with an NIR PMT or up to 2025 nm with an InGaAs detector. These upgrades maintain the standard photon-counting PMT for high sensitivity in the UV-Visible range.

Figure 1: Absorption and emission spectrum of PbS quantum dots in tetrachloroethylene measured in an FS5 with InGaAs-1650 NIR detector (NIRA1650 upgrade).

Kinetic absorption and emission scans are available in the standard FS5 Spectrofluorometer. This capability can be expanded further with a stopped flow accessory for rapid injection of reactants.

Figure 2: Bleaching of K4[Fe(CN)6] by Ascorbic Acid using the stopped-flow accessory. The absorption kinetic of Fe(CN)63- bleaching was recorded using the transmission detector of the FS5.

The SC-41 plate reader module allows measuring liquid samples with microplates up to 384 wells. As with all sample holder accessories, this microplate reader is compatible with FS5 upgrade options such as NIR detection and time-resolved photoluminescence.

Figure 3: Live measurement display for the SC-41 plate reader

If the FS5 is equipped with the polariser (POL) upgrade, automated measurements of fluorescence anisotropy are possible. A software wizard in Fluoracle guides the user through the acquisition and analysis of spectral and time-resolved (if equipped with TCSPC) anisotropy.

Figure 4: Fluorescence Anisotropy measurement of Rhodamine 6G in glycerol at room temperature (top) and anisotropy result (bottom).

Fluoracle features user-friendly wizards for acquiring and fitting calibration curves, perfect for developing quantitative analysis methods. Peak signals, areas, and ratios can be fitted to linear and non-linear curves.

Figure 5: Ratio trend analysis using Fluoracle.

CUSTOMER TESTIMONIALS

OPTIONS AND UPGRADES

A range of upgrades for NIR spectral coverage set the FS5 apart from other benchtop spectrofluorometers. A second detector is fitted to expand the operating spectral range without sacrificing the performance in the standard range. There are several versions available:

PMT-UC includes an extra cooled PMT and grating optimised for sensitivity up to 1010 nm.

NIR-TE adds a TE-cooled photon-counting NIR PMT and grating with sensitivity from 950 nm up to 1400 nm or 1650 nm.