Luminosa

Application & Methods Luminosa Single Photon Counting Confocal Microscope DESCRIPTION MATCHING APPLICATIONS & METHODS WHAT CUSTOMERS SAY DETAILS AND EXAMPLES KEY BENEFITS Explore New Paths in Confocal Microscopy Designed for Quantitative Single-Molecule Research Inspired for demanding single-molecule research, Luminosa combines precision engineering and streamlined workflows to deliver reproducible, high-quality data with exceptional sensitivity. With a big selection of excitation sources from 375 to 1064 nm and available detectors (HybridPMTs, point SPAD, SPAD array) and full access to every optomechanical component via software, this confocal microscope offers full control and flexibility for scientists at every level, accelerating results and elevating the standard of confocal imaging. Precision Designed for Single-Photon Fluorescence Built around a powerful inverted confocal fluorescence microscope, Luminosa delivers exceptional sensitivity. Its architecture combines the flexibility and precision that modern life science demands. Optimized detection: up to six parallel channels with SPADs or hybrid PMTs capture every photon with unmatched precision. Flexible scanning options: ultra-fast galvo mirrors for fast imaging or piezo objective control for maximum photon detection efficiency. High-throughput time tagging & TCSPC electronics: less than 700 ps dead time per channel and timing precision enable high-speed, high-fidelity lifetime imaging. Variable PSF: Match the confocal volume to your assay for smFRET and FCS studies LumiPy: A Python interface for customizable, adaptive workflows. PDA-23 array: An integrated SPAD array with full time-tagging. PDA-23 Detection: Confocal Time-Resolved Detection with a SPAD Array The PDA-23 SPAD array extends Luminosa with true multi-pixel, time-resolved detection at the single-photon level. By combining parallel photon counting with precise timing, it enables enhanced resolution and contrast in image scanning microscopy (ISM) while preserving quantitative fluorescence lifetime information. This makes PDA-23 a powerful upgrade for advanced confocal, FLIM, ISM applications and even the development of new methodologies for FCS and single molecule fluorescence. Analyze. Visualize. Understand. In combination with NovaFLIM and NovaISM, Luminosa extends beyond conventional confocal imaging. Advanced FLIM, FLIM-FRET and anisotropy imaging but also pixel-reassignment strategies combined with computation sectioning and deconvolution for a complete ISM-FLIM analysis workflow. This powerful and modular software combination addresses demanding applications while it offers transparent data handling, open data formats and extended metadata. Metabolic FLIM in Practice: Luminosa in Oxford Label-free metabolic FLIM offers a powerful window into tissue physiology, as demonstrated in recent work by Professor Marco Fritzsche and his team. Using Luminosa, they quantified NADH and FAD lifetimes to distinguish metabolic states in liver and intestinal tissue, revealing gradients and disease-relevant patterns. These insights highlight Luminosa’s value for Bioimaging Core Facilities seeking accessible, quantitative, time-resolved fluorescence microscopy. Read the customer story. Highlight Benefits Quality and Precision Save Time, Focus on Samples Advanced Flexibility Single Molecule Workshop Luminosa New Features Matching Applications & Methods​ Liquid-Liquid Phase Separation (LLPS) Dynamic Structural Biology Environmental Sensing Experience Luminosa What Researchers Say Latest Blog Articles Expanding the Horizon of FCS with SPAD Arrays New Analysis Options Push the Limits of FLIM Imaging Modalities Overlapping Import and Export in the Nuclear Pore Intelligent Software for Confident Results Luminosa’s advanced software suite offers intuitive, powerful tools that streamline single-molecule and fluorescence lifetime imaging experiments. With automated setup and customizable workflows, researchers can focus entirely on their samples while the software optimizes lasers, beam paths, detection, and key measurement parameters. Real-time previews and fast GPU-accelerated analysis enable instant data assessment, ensuring reproducible results and saving valuable instrument time. InstaFLIM InstaFLIM delivers real-time fluorescence lifetime analysis with minimal user interaction, enabling rapid insights and reproducible results. Four parallel routines: multi-exponential fit, phasor plot, pattern matching, lifetime histogram Automatic suggestions for FLIM species separation Suggested parameters can be fine-tuned InstaFCS InstaFCS enables real-time fluorescence correlation spectroscopy (FCS) with automated fitting, providing rapid insight into molecular dynamics. Live calculation of auto- and cross-correlation curves Automatic model and parameter suggestions Includes standard deviation of correlation curves FRETcompass FRETcompass guides researchers through complex FRET data analysis, ensuring accurate and reproducible results with minimal effort. Automated evaluation and mapping of FRET efficiencies Workflow support for parameter selection and validation Visual tools for intuitive interpretation of results LumiFinder