MicroTime 200

Application & Methods MicroTime 200 Modular Confocal Microscope with Single Molecule Sensitivity DESCRIPTION MATCHING APPLICATIONS & METHODS WHAT CUSTOMERS SAY DETAILS AND EXAMPLES KEY BENEFITS Redefining Openness and Flexibility in Confocal Microscopy Designed Around Your Research Questions to Enable New Experimental Designs Rather than being a “black box”, MicroTime 200 gives researchers the freedom and transparency to define their own approach. Its open architecture evolves with experimental needs, ensuring consistent performance, reliable results, and lasting value in cutting-edge research. Proven Reliability for Long-Term Research The most effective measure for increased sustainability is the increase lifetime of the purchased device. Built to last: robust opto-mechanics, stable alignment, long component lifecycles. Built it as your needs grow: add channels, detectors, or lasers as your research evolves. Stay always state of the art: extend system lifetime with modular swaps instead of full replacements. Expert partnership: scientific support, training, and method onboarding throughout the system’s life. Open Super-Resolution Imaging with STED The MicroTime 200 STED add-on enables time-resolved super-resolution imaging with spatial resolution below 50 nm. A STED module fitting to the open design of MicroTime 200 designed for robust, alignment-free operation. It combines the easySTED concept with fast galvo scanning to reduce phototoxicity and bleaching. Time-gated detection improves contrast and background suppression, extending super-resolution imaging to demanding fluorescence lifetime and FCS studies. Analyze. Visualize. Understand. SymPhoTime 64 provides full control over data acquisition and standard analysis workflows for time-resolved microscopy. For streamlined FLIM, FLIM-FRET and anisotropy analysis needs data can be further loaded to, NovaFLIM which delivers accelerated analysis, modern visualization, and powerful tools for extracting quantitative insight from complex datasets.This powerful and modular software combination addresses demanding applications while it offers transparent data handling, open data formats and extended metadata. Matching Applications & Methods​ Dynamic Structural Biology Semiconductor Research Nanomaterials Research What Researchers Say Latest Blog Articles Optical Characterization of Nanomaterials When Longevity Becomes a Scientific Advantage MicroTime 200 Customization: Modular Confocal Microscopy A Modular Platform Built Around Your Experiment MicroTime 200 is designed as a flexible, modular system that adapts to a wide range of experimental requirements. Excitation, detection, and scanning can be configured independently, allowing researchers to optimize every part of the measurement chain for their specific application. A Flexible Excitation Subsystem Select from a broad range of laser sources covering 255–900 nm to match fluorophores, quantum emitters, or fluorescent proteins. Create complex excitation patterns like pulsed-interleaved excitation (PIE) for FRET or multi-wavelength sequences for contrast without redesigning your setup. Detection with Single Photon Sensitivity Mix and match up to six detection channels for maximum flexibility. Combine high-timing-precision hybrid detectors with high-QE SPADs to balance count rate and spectral coverage. Minimal afterpulsing and short dead time maintain signal integrity during fast decays, antibunching measurements, and correlation analyses in both biological and solid-state samples. Time Tagging and TCSPC Electronics Capture every photon with picosecond timing precision and high throughput. Advanced time tagging and TCSPC electronics enable accurate correlation, lifetime, and timing analysis across a wide dynamic range. Short dead times and scalable channel configurations ensure reliable measurements for both single-molecule studies and complex photonic experiments. Customized Setups for Demanding Experiments MicroTime 200 can be customized to match highly specific experimental demands, from correlated AFM–FLIM measurements and cryogenic single-molecule studies to spectrally resolved and multiphoton experiments. Explore real-world customization examples in our blog article. Premium Resources Access in-depth application notes and scientific posters with detailed methods, measurement data, and real-world use cases. APPLICATION NOTE: TIME-GATED FCS Time-gated FCS for improved background suppression and accurate concentration measurements APPLICATION NOTE: FRET ANALYSIS USING PIE FRET analysis using Pulsed Interleaved Excitation (PIE) with the MicroTime 200 for accurate single-molecule studies and improved FRET efficiency determination INFORMATION BUNDLE: FCS AND RELATED TECHNIQUES Information bundle on fluorescence correlation spectroscopy (FCS) covering quantitative FCS, liFCS, dual-focus FCS, FLCS, calibration methods, and diffusion analysis. CUSTOMER VIDEO: PROBING THE DYNAMICS AND INTERACTION MECHANISMS OF IDPS WITH SINGLE-MOLECULE SPECTROSCOPY In this customer video, Ben Schuler (University of Zürich) shows how single-molecule FRET combined with correlation spectroscopy and microfluidic mixing reveals the dynamics of intrinsically disordered proteins across timescales and environments. CUSTOMER VIDEO: MULTIMODAL MICROSCOPY OF HALIDE PEROVSKITE SOLAR CELLS In this video, Sam Stranks (University of Cambridge), gives an overview of halide perovskite solar cell research using multimodal microscopy to study luminescence, recombination, degradation, and pathways to improve efficiency and stability. CASE STUDY: THE DREAM MICROSCOPE Unlocking the Secrets of Perovskite Solar Cells with Correlative Multimodal Microscopy Expandable Through Powerful Add-Ons A range of standard add-ons extends MicroTime 200 to support diverse experimental requirements. Many of these options can be combined, allowing researchers to build powerful, application-specific configurations tailored to their lab’s needs. AFM Integration for Correlated Nanoscale Insight Combine single-molecule fluorescence with nanometer-resolved AFM measurements for structural and dynamic correlation. Flexible Spectral Selection with FlexLambda