Open FluorCam

Open FluorCam FC 800-O/1010 and FC 800-O/2020 are highly modular instruments with flexible geometry. The LED panels and the light source generating saturating flashes can be arranged at various angles and distances from the sample. Also, the position of the camera relative to the sample is adjustable.

Four super bright LED panels available in specific wavelengths

Adjustable system for different plant sizes

Supplied with a notebook and software

High-resolution CCD camera

Dark room for adaptation (optional)

Additional top stand mounted LED panel (optional)

Additional exchangeable LED panels (optional)

Open FluorCam Open FluorCam FC 800-O/1010 and FC 800-O/2020 are a highly modular instruments with flexible geometry enabling work with samples of various sizes (from microtiter plates to small trees). Open FluorCam FC 800-O represents a highly innovative, robust and user-friendly world-wide used system for combined multispectral and kinetic fluorescence imaging. It consists of a CCD camera, four to five fixed LED panels (4 + 1 additional, which is not included in the standard setup) and, optionally, of a filter wheel equipped with up to 7 different emission filters. The four LED panels can be arranged at various angles and distances from the sample. The position of the camera may also be adjusted with respect to the sample height.

The dimensions of the LED panels can be 130 × 130 mm or 200 × 200 mm, which is suitable for imaging of small plants (such as Arabidopsis thaliana) up to middle size plants or small tress, detached leaves, fruits, vegetables, etc. The system allows easy dark adaptation of an investigated sample if dark box (it is not part of the standard set-up).

The Open FluorCam FC 800-O/1010 generates images of fluorescence signal at any moment of the experiment and presents them using a false color scale. Full kinetic analysis is available. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols. The Open FluorCam FC 800-O also includes a high-performance PC and comprehensive software package comprising full system control, data acquisition and image processing. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences. Light Panels Standard version of the FluorCam system includes four super bright LED panels - size 130 x 130 mm or 200 x 200 mm. One pair of LED panels provides Measuring Light and Actinic Light 1 (red-orange 617 nm - in standard version). Other two panels provide Actinic Light 2 and Saturating Pulse (cool white 6500 K - in standard version). Other available wavelengths for light sources are: royal-blue (447 nm), blue (470 nm), green (530 nm), cyan (505 nm), red (627 nm), deep-red (655 nm), amber (590 nm) - these lights sources are mounted only in customized FluorCam versions. Additional LED panel may be added to increase the colour spectrum of the light sources for multi-colour fluorescence measurements. Additional panel is mounted around the camera. Available colours are: far-red (740 nm), deep-red (660 nm), ultra-violet (365 or 385 nm), green (523 nm), amber (590 nm). Other options available upon request. Actinic light intensity: - 300-2,000 µmol(photons)/m².s in the standard version depending on light wavelength, - up to 3,000 µmol(photons)/m².s in the light-upgraded version - please inquire at: info@psi.cz Super pulse intensity: - up to 4,000 µmol(photons)/m².s (in the standard version), - up to 6,000 µmol(photons)/m².s (in the light-upgraded version - please inquire at: info@psi.cz Single Turnover Flash (STF): 120,000 µmol(photons)/m².s in 100µs pulse (in the QA re-oxidation version) Protocols FV/FM Kautsky induction Quenching analysis Light Curve Steady state fluorescence, e.g., ChlF, GFP and other FPs (flter wheel required) Multicolor fluorescence, e.g., UV induced plant blue-green fluorescence (F440, F520) and chlorophyll fluorescence (F680, F740); flter wheel required PAR absorptivity (it needs optional accessories: flter wheel and additional far-red LED panel) Measured Parameters Measured parameters: F O , F M , F V , F O' , F M' , F V' , F T More than 50 calculated parameters: F V /F M , F V' /F M' , Phi PSII , NPQ, qN, qP, Rfd, ETR (via Light Curve protocol), PAR-absorptivity coefficient, and many others

Standard version of the FluorCam system includes four super bright LED panels - size 130 x 130 mm or 200 x 200 mm. One pair of LED panels provides Measuring Light and Actinic Light 1 (red-orange 617 nm - in standard version). Other two panels provide Actinic Light 2 and Saturating Pulse (cool white 6500 K - in standard version).

Other available wavelengths for light sources are: royal-blue (447 nm), blue (470 nm), green (530 nm), cyan (505 nm), red (627 nm), deep-red (655 nm), amber (590 nm) - these lights sources are mounted only in customized FluorCam versions.

Additional LED panel may be added to increase the colour spectrum of the light sources for multi-colour fluorescence measurements. Additional panel is mounted around the camera. Available colours are: far-red (740 nm), deep-red (660 nm), ultra-violet (365 or 385 nm), green (523 nm), amber (590 nm). Other options available upon request.

Single Turnover Flash (STF): 120,000 µmol(photons)/m².s in 100µs pulse (in the QA re-oxidation version)

Steady state fluorescence, e.g., ChlF, GFP and other FPs (flter wheel required)

Multicolor fluorescence, e.g., UV induced plant blue-green fluorescence (F440, F520) and chlorophyll fluorescence (F680, F740); flter wheel required

PAR absorptivity (it needs optional accessories: flter wheel and additional far-red LED panel)

Measured parameters: F O , F M , F V , F O' , F M' , F V' , F T

More than 50 calculated parameters: F V /F M , F V' /F M' , Phi PSII , NPQ, qN, qP, Rfd, ETR (via Light Curve protocol), PAR-absorptivity coefficient, and many others

Screening for photosynthetic performance and metabolic perturbations

Detection of biotic and abiotic stress

Plant’s resistance or susceptibility to various stress factors

Agriculture and horticulture